The following is a list of frequently requested telephone numbers. If you can't find what you are looking for, please call Brooksville Regional Hospital's main switchboard at (352) 796-5111. For patient names and room numbers, please contact the main switchboard for further assistance.
| Department | Phone Number |
| Billing / Business Office | (352) 799-4001 |
| Administration | (352) 544-6020 |
| Admissions | (352) 544-6065 |
| Accounting | (352) 544-6130 |
| Cardiopulmonary Services | (352) 544-6017 |
| Case Management | (352) 544-6086 |
| Central Diagnostic Scheduling | (352) 754-3231 |
| Dietary Services | (352) 544-6109 |
| Education | (352) 544-6144 |
| Emergency Department (dial 911 in an emergency) | (352) 544-6060 |
| Employee Health Nurse | (352) 544-6088 |
| Environmental Resources | (352) 544-6055 |
| Human Resources | (352) 544-6150 |
| ICU Extension Unit | (352) 754-3286 |
| Infection Control | (352) 797-4650 |
| Intensive Care Unit | (352) 544-6010 |
| Joint Center | (352) 544-6001 |
| Laboratory | (352) 544-6043 |
| Maintenance | (352) 544-6057 |
| Materials Management | (352) 544-6101 |
| Medical Records | (352) 544-6093 |
| Medical Surgical Unit (3rd Floor) | (352) 544-6112 |
| PCU - Progressive Care Unit (2nd Floor) | (352) 544-6125 |
| Pharmacy | (352) 544-6075 |
| Radiology | (352) 544-6105 |
| Rehabilitation Services | (352) 544-6028 |
| Risk Management | (352) 544-6009 |
| Same-Day Surgery Unit | (352) 544-6003 |
| Surgery Department | (352) 544-6016 |
| Surgical Scheduling | (352) 797-4601 |
| Volunteer Services | (352) 544-6117 |
| Warehouse | (352) 754-3274 |